Control:Article Title: The role of small extracellular vesicle-miRNAs in endometriosis
Article Snippet: .. 3 , Serum Eutopic endometrium Endometriotic lesion Endometriosis mouse model , Human samples : Endometriosis (ovarian endometrioma, from ovarian cystectomy or oopherectomy and confirmed with histology) and control (hysterectomy for other pelvic masses): *Serum, n=20 each Eutopic endometrium, n=unclear Endometriotic lesion, n=unclear *sEV source **Proliferative phase samples Samples from mice : Endometriosis mouse model, n=24, divided into 4 treatment groups , ExoQuick-TC Exosome Isolation Kit (System Biosciences SBI, USA) , TEM: 90–120 nm. NTA not done. , WB: CD9 and CD63 positive. , Immunohistochemistry and WB for CCN2, α-SMA, and collagen α1. miRNA extraction of all cell types and qRT-PCR for hsa-miR-214-3p. Transfection of all cell types with hsa-miR-214-3p/mimics/NC and qRT-PCR for miRNA and CCN2. Co-culture of transfected and untransfected cells followed by qRT-PCR for miRNA and CCN2. Serum sEV-miRNA: qRT-PCR . Endometriosis mouse model: sEV-miRNA uptake experiment , qRT-PCR . , ↓ hsa-miR-214-3p , Zhang et al. (2021) . .. 4 , Plasma Eutopic endometrium Endometriotic lesion PF , Human samples : Endometriosis (stages III–IV, diagnosed via laparoscopy): Plasma, n=6 Eutopic endometrium, n=6 Endometriotic lesion, n=6 PF, n=6 Control (free from endometriosis, infertility, and gynaecological malignancies): No other information given *Matched, secretory phase samples , MiRCURY Exosome Isolation Kit (Qiagen) , TEM : 30–150 nm. NTA not done. , WB: CD63 positive, calnexin negative. , sEV-miRNA sequencing of all sample types followed by qRT-PCR . Proteomics experiments (out of review scope). sEV uptake experiments by endometriotic lesion/eutopic endometrium-endothelial co-culture. , ↑ hsa-miR-27a-3p ↓ hsa-miR-30d-5p, hsa-miR-375 Regardless of sample types , Khalaj et al. (2019) .
Mouse Assay:Article Title: The role of small extracellular vesicle-miRNAs in endometriosis
Article Snippet: .. 3 , Serum Eutopic endometrium Endometriotic lesion Endometriosis mouse model , Human samples : Endometriosis (ovarian endometrioma, from ovarian cystectomy or oopherectomy and confirmed with histology) and control (hysterectomy for other pelvic masses): *Serum, n=20 each Eutopic endometrium, n=unclear Endometriotic lesion, n=unclear *sEV source **Proliferative phase samples Samples from mice : Endometriosis mouse model, n=24, divided into 4 treatment groups , ExoQuick-TC Exosome Isolation Kit (System Biosciences SBI, USA) , TEM: 90–120 nm. NTA not done. , WB: CD9 and CD63 positive. , Immunohistochemistry and WB for CCN2, α-SMA, and collagen α1. miRNA extraction of all cell types and qRT-PCR for hsa-miR-214-3p. Transfection of all cell types with hsa-miR-214-3p/mimics/NC and qRT-PCR for miRNA and CCN2. Co-culture of transfected and untransfected cells followed by qRT-PCR for miRNA and CCN2. Serum sEV-miRNA: qRT-PCR . Endometriosis mouse model: sEV-miRNA uptake experiment , qRT-PCR . , ↓ hsa-miR-214-3p , Zhang et al. (2021) . .. 4 , Plasma Eutopic endometrium Endometriotic lesion PF , Human samples : Endometriosis (stages III–IV, diagnosed via laparoscopy): Plasma, n=6 Eutopic endometrium, n=6 Endometriotic lesion, n=6 PF, n=6 Control (free from endometriosis, infertility, and gynaecological malignancies): No other information given *Matched, secretory phase samples , MiRCURY Exosome Isolation Kit (Qiagen) , TEM : 30–150 nm. NTA not done. , WB: CD63 positive, calnexin negative. , sEV-miRNA sequencing of all sample types followed by qRT-PCR . Proteomics experiments (out of review scope). sEV uptake experiments by endometriotic lesion/eutopic endometrium-endothelial co-culture. , ↑ hsa-miR-27a-3p ↓ hsa-miR-30d-5p, hsa-miR-375 Regardless of sample types , Khalaj et al. (2019) .
Isolation:Article Title: The role of small extracellular vesicle-miRNAs in endometriosis
Article Snippet: .. 3 , Serum Eutopic endometrium Endometriotic lesion Endometriosis mouse model , Human samples : Endometriosis (ovarian endometrioma, from ovarian cystectomy or oopherectomy and confirmed with histology) and control (hysterectomy for other pelvic masses): *Serum, n=20 each Eutopic endometrium, n=unclear Endometriotic lesion, n=unclear *sEV source **Proliferative phase samples Samples from mice : Endometriosis mouse model, n=24, divided into 4 treatment groups , ExoQuick-TC Exosome Isolation Kit (System Biosciences SBI, USA) , TEM: 90–120 nm. NTA not done. , WB: CD9 and CD63 positive. , Immunohistochemistry and WB for CCN2, α-SMA, and collagen α1. miRNA extraction of all cell types and qRT-PCR for hsa-miR-214-3p. Transfection of all cell types with hsa-miR-214-3p/mimics/NC and qRT-PCR for miRNA and CCN2. Co-culture of transfected and untransfected cells followed by qRT-PCR for miRNA and CCN2. Serum sEV-miRNA: qRT-PCR . Endometriosis mouse model: sEV-miRNA uptake experiment , qRT-PCR . , ↓ hsa-miR-214-3p , Zhang et al. (2021) . .. 4 , Plasma Eutopic endometrium Endometriotic lesion PF , Human samples : Endometriosis (stages III–IV, diagnosed via laparoscopy): Plasma, n=6 Eutopic endometrium, n=6 Endometriotic lesion, n=6 PF, n=6 Control (free from endometriosis, infertility, and gynaecological malignancies): No other information given *Matched, secretory phase samples , MiRCURY Exosome Isolation Kit (Qiagen) , TEM : 30–150 nm. NTA not done. , WB: CD63 positive, calnexin negative. , sEV-miRNA sequencing of all sample types followed by qRT-PCR . Proteomics experiments (out of review scope). sEV uptake experiments by endometriotic lesion/eutopic endometrium-endothelial co-culture. , ↑ hsa-miR-27a-3p ↓ hsa-miR-30d-5p, hsa-miR-375 Regardless of sample types , Khalaj et al. (2019) .
Transmission Electron Microscopy:Article Title: The role of small extracellular vesicle-miRNAs in endometriosis
Article Snippet: .. 3 , Serum Eutopic endometrium Endometriotic lesion Endometriosis mouse model , Human samples : Endometriosis (ovarian endometrioma, from ovarian cystectomy or oopherectomy and confirmed with histology) and control (hysterectomy for other pelvic masses): *Serum, n=20 each Eutopic endometrium, n=unclear Endometriotic lesion, n=unclear *sEV source **Proliferative phase samples Samples from mice : Endometriosis mouse model, n=24, divided into 4 treatment groups , ExoQuick-TC Exosome Isolation Kit (System Biosciences SBI, USA) , TEM: 90–120 nm. NTA not done. , WB: CD9 and CD63 positive. , Immunohistochemistry and WB for CCN2, α-SMA, and collagen α1. miRNA extraction of all cell types and qRT-PCR for hsa-miR-214-3p. Transfection of all cell types with hsa-miR-214-3p/mimics/NC and qRT-PCR for miRNA and CCN2. Co-culture of transfected and untransfected cells followed by qRT-PCR for miRNA and CCN2. Serum sEV-miRNA: qRT-PCR . Endometriosis mouse model: sEV-miRNA uptake experiment , qRT-PCR . , ↓ hsa-miR-214-3p , Zhang et al. (2021) . .. 4 , Plasma Eutopic endometrium Endometriotic lesion PF , Human samples : Endometriosis (stages III–IV, diagnosed via laparoscopy): Plasma, n=6 Eutopic endometrium, n=6 Endometriotic lesion, n=6 PF, n=6 Control (free from endometriosis, infertility, and gynaecological malignancies): No other information given *Matched, secretory phase samples , MiRCURY Exosome Isolation Kit (Qiagen) , TEM : 30–150 nm. NTA not done. , WB: CD63 positive, calnexin negative. , sEV-miRNA sequencing of all sample types followed by qRT-PCR . Proteomics experiments (out of review scope). sEV uptake experiments by endometriotic lesion/eutopic endometrium-endothelial co-culture. , ↑ hsa-miR-27a-3p ↓ hsa-miR-30d-5p, hsa-miR-375 Regardless of sample types , Khalaj et al. (2019) .
Western Blot:Article Title: The role of small extracellular vesicle-miRNAs in endometriosis
Article Snippet: .. 3 , Serum Eutopic endometrium Endometriotic lesion Endometriosis mouse model , Human samples : Endometriosis (ovarian endometrioma, from ovarian cystectomy or oopherectomy and confirmed with histology) and control (hysterectomy for other pelvic masses): *Serum, n=20 each Eutopic endometrium, n=unclear Endometriotic lesion, n=unclear *sEV source **Proliferative phase samples Samples from mice : Endometriosis mouse model, n=24, divided into 4 treatment groups , ExoQuick-TC Exosome Isolation Kit (System Biosciences SBI, USA) , TEM: 90–120 nm. NTA not done. , WB: CD9 and CD63 positive. , Immunohistochemistry and WB for CCN2, α-SMA, and collagen α1. miRNA extraction of all cell types and qRT-PCR for hsa-miR-214-3p. Transfection of all cell types with hsa-miR-214-3p/mimics/NC and qRT-PCR for miRNA and CCN2. Co-culture of transfected and untransfected cells followed by qRT-PCR for miRNA and CCN2. Serum sEV-miRNA: qRT-PCR . Endometriosis mouse model: sEV-miRNA uptake experiment , qRT-PCR . , ↓ hsa-miR-214-3p , Zhang et al. (2021) . .. 4 , Plasma Eutopic endometrium Endometriotic lesion PF , Human samples : Endometriosis (stages III–IV, diagnosed via laparoscopy): Plasma, n=6 Eutopic endometrium, n=6 Endometriotic lesion, n=6 PF, n=6 Control (free from endometriosis, infertility, and gynaecological malignancies): No other information given *Matched, secretory phase samples , MiRCURY Exosome Isolation Kit (Qiagen) , TEM : 30–150 nm. NTA not done. , WB: CD63 positive, calnexin negative. , sEV-miRNA sequencing of all sample types followed by qRT-PCR . Proteomics experiments (out of review scope). sEV uptake experiments by endometriotic lesion/eutopic endometrium-endothelial co-culture. , ↑ hsa-miR-27a-3p ↓ hsa-miR-30d-5p, hsa-miR-375 Regardless of sample types , Khalaj et al. (2019) .
Immunohistochemistry:Article Title: The role of small extracellular vesicle-miRNAs in endometriosis
Article Snippet: .. 3 , Serum Eutopic endometrium Endometriotic lesion Endometriosis mouse model , Human samples : Endometriosis (ovarian endometrioma, from ovarian cystectomy or oopherectomy and confirmed with histology) and control (hysterectomy for other pelvic masses): *Serum, n=20 each Eutopic endometrium, n=unclear Endometriotic lesion, n=unclear *sEV source **Proliferative phase samples Samples from mice : Endometriosis mouse model, n=24, divided into 4 treatment groups , ExoQuick-TC Exosome Isolation Kit (System Biosciences SBI, USA) , TEM: 90–120 nm. NTA not done. , WB: CD9 and CD63 positive. , Immunohistochemistry and WB for CCN2, α-SMA, and collagen α1. miRNA extraction of all cell types and qRT-PCR for hsa-miR-214-3p. Transfection of all cell types with hsa-miR-214-3p/mimics/NC and qRT-PCR for miRNA and CCN2. Co-culture of transfected and untransfected cells followed by qRT-PCR for miRNA and CCN2. Serum sEV-miRNA: qRT-PCR . Endometriosis mouse model: sEV-miRNA uptake experiment , qRT-PCR . , ↓ hsa-miR-214-3p , Zhang et al. (2021) . .. 4 , Plasma Eutopic endometrium Endometriotic lesion PF , Human samples : Endometriosis (stages III–IV, diagnosed via laparoscopy): Plasma, n=6 Eutopic endometrium, n=6 Endometriotic lesion, n=6 PF, n=6 Control (free from endometriosis, infertility, and gynaecological malignancies): No other information given *Matched, secretory phase samples , MiRCURY Exosome Isolation Kit (Qiagen) , TEM : 30–150 nm. NTA not done. , WB: CD63 positive, calnexin negative. , sEV-miRNA sequencing of all sample types followed by qRT-PCR . Proteomics experiments (out of review scope). sEV uptake experiments by endometriotic lesion/eutopic endometrium-endothelial co-culture. , ↑ hsa-miR-27a-3p ↓ hsa-miR-30d-5p, hsa-miR-375 Regardless of sample types , Khalaj et al. (2019) .
Extraction:Article Title: The role of small extracellular vesicle-miRNAs in endometriosis
Article Snippet: .. 3 , Serum Eutopic endometrium Endometriotic lesion Endometriosis mouse model , Human samples : Endometriosis (ovarian endometrioma, from ovarian cystectomy or oopherectomy and confirmed with histology) and control (hysterectomy for other pelvic masses): *Serum, n=20 each Eutopic endometrium, n=unclear Endometriotic lesion, n=unclear *sEV source **Proliferative phase samples Samples from mice : Endometriosis mouse model, n=24, divided into 4 treatment groups , ExoQuick-TC Exosome Isolation Kit (System Biosciences SBI, USA) , TEM: 90–120 nm. NTA not done. , WB: CD9 and CD63 positive. , Immunohistochemistry and WB for CCN2, α-SMA, and collagen α1. miRNA extraction of all cell types and qRT-PCR for hsa-miR-214-3p. Transfection of all cell types with hsa-miR-214-3p/mimics/NC and qRT-PCR for miRNA and CCN2. Co-culture of transfected and untransfected cells followed by qRT-PCR for miRNA and CCN2. Serum sEV-miRNA: qRT-PCR . Endometriosis mouse model: sEV-miRNA uptake experiment , qRT-PCR . , ↓ hsa-miR-214-3p , Zhang et al. (2021) . .. 4 , Plasma Eutopic endometrium Endometriotic lesion PF , Human samples : Endometriosis (stages III–IV, diagnosed via laparoscopy): Plasma, n=6 Eutopic endometrium, n=6 Endometriotic lesion, n=6 PF, n=6 Control (free from endometriosis, infertility, and gynaecological malignancies): No other information given *Matched, secretory phase samples , MiRCURY Exosome Isolation Kit (Qiagen) , TEM : 30–150 nm. NTA not done. , WB: CD63 positive, calnexin negative. , sEV-miRNA sequencing of all sample types followed by qRT-PCR . Proteomics experiments (out of review scope). sEV uptake experiments by endometriotic lesion/eutopic endometrium-endothelial co-culture. , ↑ hsa-miR-27a-3p ↓ hsa-miR-30d-5p, hsa-miR-375 Regardless of sample types , Khalaj et al. (2019) .
Quantitative RT-PCR:Article Title: The role of small extracellular vesicle-miRNAs in endometriosis
Article Snippet: .. 3 , Serum Eutopic endometrium Endometriotic lesion Endometriosis mouse model , Human samples : Endometriosis (ovarian endometrioma, from ovarian cystectomy or oopherectomy and confirmed with histology) and control (hysterectomy for other pelvic masses): *Serum, n=20 each Eutopic endometrium, n=unclear Endometriotic lesion, n=unclear *sEV source **Proliferative phase samples Samples from mice : Endometriosis mouse model, n=24, divided into 4 treatment groups , ExoQuick-TC Exosome Isolation Kit (System Biosciences SBI, USA) , TEM: 90–120 nm. NTA not done. , WB: CD9 and CD63 positive. , Immunohistochemistry and WB for CCN2, α-SMA, and collagen α1. miRNA extraction of all cell types and qRT-PCR for hsa-miR-214-3p. Transfection of all cell types with hsa-miR-214-3p/mimics/NC and qRT-PCR for miRNA and CCN2. Co-culture of transfected and untransfected cells followed by qRT-PCR for miRNA and CCN2. Serum sEV-miRNA: qRT-PCR . Endometriosis mouse model: sEV-miRNA uptake experiment , qRT-PCR . , ↓ hsa-miR-214-3p , Zhang et al. (2021) . .. 4 , Plasma Eutopic endometrium Endometriotic lesion PF , Human samples : Endometriosis (stages III–IV, diagnosed via laparoscopy): Plasma, n=6 Eutopic endometrium, n=6 Endometriotic lesion, n=6 PF, n=6 Control (free from endometriosis, infertility, and gynaecological malignancies): No other information given *Matched, secretory phase samples , MiRCURY Exosome Isolation Kit (Qiagen) , TEM : 30–150 nm. NTA not done. , WB: CD63 positive, calnexin negative. , sEV-miRNA sequencing of all sample types followed by qRT-PCR . Proteomics experiments (out of review scope). sEV uptake experiments by endometriotic lesion/eutopic endometrium-endothelial co-culture. , ↑ hsa-miR-27a-3p ↓ hsa-miR-30d-5p, hsa-miR-375 Regardless of sample types , Khalaj et al. (2019) .
Transfection:Article Title: The role of small extracellular vesicle-miRNAs in endometriosis
Article Snippet: .. 3 , Serum Eutopic endometrium Endometriotic lesion Endometriosis mouse model , Human samples : Endometriosis (ovarian endometrioma, from ovarian cystectomy or oopherectomy and confirmed with histology) and control (hysterectomy for other pelvic masses): *Serum, n=20 each Eutopic endometrium, n=unclear Endometriotic lesion, n=unclear *sEV source **Proliferative phase samples Samples from mice : Endometriosis mouse model, n=24, divided into 4 treatment groups , ExoQuick-TC Exosome Isolation Kit (System Biosciences SBI, USA) , TEM: 90–120 nm. NTA not done. , WB: CD9 and CD63 positive. , Immunohistochemistry and WB for CCN2, α-SMA, and collagen α1. miRNA extraction of all cell types and qRT-PCR for hsa-miR-214-3p. Transfection of all cell types with hsa-miR-214-3p/mimics/NC and qRT-PCR for miRNA and CCN2. Co-culture of transfected and untransfected cells followed by qRT-PCR for miRNA and CCN2. Serum sEV-miRNA: qRT-PCR . Endometriosis mouse model: sEV-miRNA uptake experiment , qRT-PCR . , ↓ hsa-miR-214-3p , Zhang et al. (2021) . .. 4 , Plasma Eutopic endometrium Endometriotic lesion PF , Human samples : Endometriosis (stages III–IV, diagnosed via laparoscopy): Plasma, n=6 Eutopic endometrium, n=6 Endometriotic lesion, n=6 PF, n=6 Control (free from endometriosis, infertility, and gynaecological malignancies): No other information given *Matched, secretory phase samples , MiRCURY Exosome Isolation Kit (Qiagen) , TEM : 30–150 nm. NTA not done. , WB: CD63 positive, calnexin negative. , sEV-miRNA sequencing of all sample types followed by qRT-PCR . Proteomics experiments (out of review scope). sEV uptake experiments by endometriotic lesion/eutopic endometrium-endothelial co-culture. , ↑ hsa-miR-27a-3p ↓ hsa-miR-30d-5p, hsa-miR-375 Regardless of sample types , Khalaj et al. (2019) .
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